Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LRP2BP / LRP2 Binding Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111758-100
Catalog Number: LS-G111758
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRP2BP (NM_018409), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MegBP | LRP2 binding protein | Megalin-binding protein | KIAA1325 | LRP2-binding protein | LRP2BP
- Origin Species
- Human
- NCBI GenBank
- NM_018409
- SwissProt
- Q9P2M1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKLTSEKLPK NPFYASVSQY AAKNQKFFQW KKEKTDYTHA NLVDKALQLL KERILKGDTL61AYFLRGQLYF EEGWYEEALE QFEEIKEKDH QATYQLGVMY YDGLGTTLDA EKGVDYMKKI121LDSPCPKARH LKFAAAYNLG RAYYEGKGVK RSNEEAERLW LIAADNGNPK ASVKAQSMLG181LYYSTKEPKE LEKAFYWHSE ACGNGNLESQ GALGLMYLYG QGIRQDTEAA LQCLREAAER241GNVYAQGNLV EYYYKMKFFT KCVAFSKRIA DYDEVHDIPM IAQVTDCLPE FIGRGMAMAS301FYHARCLQLG LGITRDETTA KHYYSKACRL NPALADELHS LLIRQRITRTRPLEQKLISEEDLAANDILDYKDDDDKV
347 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.