LSBio
LRP10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRP10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRP10 antibodies →
$725.00each
Catalog Number: LS-G114171-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Low density lipoprotein receptor related protein 10(LRP10) (NM_014045), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP564C1940 | LRP-10 | LRP10 | LRP9 | MST087 | MSTP087
Origin Species
Human
NCBI GenBank
NM_014045
SwissProt
Q7Z4F1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.19 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLATLLLLL LGGALAHPDR IIFPNHACED PPAVLLEVQG TLQRPLVRDS RTSPANCTWL
61ILGSKEQTVT IRFQKLHLAC GSERLTLRSP LQPLISLCEA PPSPLQLPGG NVTITYSYAG
121ARAPMGQGFL LSYSQDWLMC LQEEFQCLNH RCVSAVQRCD GVDACGDGSD EAGCSSDPFP
181GLTPRPVPSL PCNVTLEDFY GVFSSPGYTH LASVSHPQSC HWLLDPHDGR RLAVRFTALD
241LGFGDAVHVY DGPGPPESSR LLRSLTHFSN GKAVTVETLS GQAVVSYHTV AWSNGRGFNA
301TYHVRGYCLP WDRPCGLGSG LGAGEGLGER CYSEAQRCDG SWDCADGTDE EDCPGCPPGH
361FPCGAAGTSG ATACYLPADR CNYQTFCADG ADERRCRHCQ PGNFRCRDEK CVYETWVCDG
421QPDCADGSDE WDCSYVLPRK VITAAVIGSL VCGLLLVIAL GCTCKLYAIR TQEYSIFAPL
481SRMEAEIVQQ QAPPSYGQLI AQGAIPPVED FPTENPNDNS VLGNLRSLLQ ILRQDMTPGG
541GPGARRRQRG RLMRRLVRRL RRWGLLPRTN TPARASEARS QVTPSAAPLE ALDGGTGPAR
601EGGAVGGQDG EQAPPLPIKA PLPSASTSPA PTTVPEAPGP LPSLPLEPSL LSGVVQALRG
661RLLPSLGPPG PTRSPPGPHT AVLALEDEDD VLLVPLAEPG VWVAEAEDEP LLT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

713 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.