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LRIT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRIT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRIT2 antibodies →
$725.00each
Catalog Number: LS-G121288-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRIT2 (NM_001017924), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001284223

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60 kda
Protein Type
Lysate
Amino Acid Sequence
1MASVFHYFLL VLVFLDTHAA QPFCLPGCTC SEESFGRTLQ CTSVSLGKIP GNLSEEFKQV
61RIENSPLFEM PQGSFINMST LEYLWLNFNN ISVIHLGALE HLPELRELRL EGNKLCSVPW
121TAFRATPLLR VLDLKRNKID ALPELALQFL VSLTYLDLSS NRLTVVSKSV FLNWPAYQKC
181RQPDCGAEIL SSLVVALHDN PWVCDCRLRG LVQFVKSITL PVILVNSYLI CQGPLSKAGQ
241LFHETELSAC MKPQISTPSA NITIRAGQNV TLRCLAQASP SPSIAWTYPL SMWREFDVLT
301SSTGEDTALS ELAIPAAHLV DSGNYTCMAS NSIGKSNLVI SLHVQPAQAL HAPDSLSIPS
361EGNAYIDLRV VKQTVHGILL EWLAVADTSK EEWFTLYIAS DEAFRKEVVH IGPGINTYAV
421DDLLPGTKYE ACLSLEGQPP HQGQCVAFVT GRDAGGLEAR EHLLHVTVVL CVVLLAVPVG
481AYAWAAQGPC SCSKWVLRGC LHRRKAPSCT PAAPQSKDGS FREHPAVCDD GEGHIDTEGD
541KEKGGTEDNS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

550 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.