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LRIG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRIG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105818-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRIG3 (NM_153377), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
123.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAPSLRARA AGLGLLLCAV LGRAGRSDSG GRGELGQPSG VAAERPCPTT CRCLGDLLDC
61SRKRLARLPE PLPSWVARLD LSHNRLSFIK ASSMSHLQSL REVKLNNNEL ETIPNLGPVS
121ANITLLSLAG NRIVEILPEH LKEFQSLETL DLSSNNISEL QTAFPALQLK YLYLNSNRVT
181SMEPGYFDNL ANTLLVLKLN RNRISAIPPK MFKLPQLQHL ELNRNKIKNV DGLTFQGLGA
241LKSLKMQRNG VTKLMDGAFW GLSNMEILQL DHNNLTEITK GWLYGLLMLQ ELHLSQNAIN
301RISPDAWEFC QKLSELDLTF NHLSRLDDSS FLGLSLLNTL HIGNNRVSYI ADCAFRGLSS
361LKTLDLKNNE ISWTIEDMNG AFSGLDKLRR LILQGNRIRS ITKKAFTGLD ALEHLDLSDN
421AIMSLQGNAF SQMKKLQQLH LNTSSLLCDC QLKWLPQWVA ENNFQSFVNA SCAHPQLLKG
481RSIFAVSPDG FVCDDFPKPQ ITVQPETQSA IKGSNLSFIC SAASSSDSPM TFAWKKDNEL
541LHDAEMENYA HLRAQGGEVM EYTTILRLRE VEFASEGKYQ CVISNHFGSS YSVKAKLTVN
601MLPSFTKTPM DLTIRAGAMA RLECAAVGHP APQIAWQKDG GTDFPAARER RMHVMPEDDV
661FFIVDVKIED IGVYSCTAQN SAGSISANAT LTVLETPSFL RPLLDRTVTK GETAVLQCIA
721GGSPPPKLNW TKDDSPLVVT ERHFFAAGNQ LLIIVDSDVS DAGKYTCEMS NTLGTERGNV
781RLSVIPTPTC DSPQMTAPSL DDDGWATVGV VIIAVVCCVV GTSLVWVVII YHTRRRNEDC
841SITNTDETNL PADIPSYLSS QGTLADRQDG YVSSESGSHH QFVTSSGAGF FLPQHDSSGT
901CHIDNSSEAD VEAATDLFLC PFLGSTGPMY LKGNVYGSDP FETYHTGCSP DPRTVLMDHY
961EPSYIKKKEC YPCSHPSEES CERSFSNISW PSHVRKLLNT SYSHNEGPGM KNLCLNKSSL
1021DFSANPEPAS VASSNSFMGT FGKALRRPHL DAYSSFGQPS DCQPRAFYLK AHSSPDLDSG
1081SEEDGKERTD FQEENHICTF KQTLENYRTP NFQSYDLDT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1119 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.