LSBio
LRGUK Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRGUK Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRGUK antibodies →
$504.00each
Catalog Number: LS-G106903-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRGUK (NM_144648), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MATSERALLR TRAASLLRGL GRSRTGARSL QFRAEKERQP CWSFPMGQKT KGSSNIASSY
61LLQQLMHRYQ ELDSDGDEDQ GEGEAGSEES SESEMLNLEE EFDGVLREEA VAKALHHLGR
121SGSGTEQVYL NLTLSGCNLI DVSILCGYVH LQKLDLSANK IEDLSCVSCM PYLLELNASQ
181NNLTTFFNFK PPKNLKKADF SHNQISEICD LSAYHALTKL ILDGNEIEEI SGLEMCNNLI
241HLSLANNKIT TINGLNKLPI KILCLSNNQI EMITGLEDLK ALQNLDLSHN QISSLQGLEN
301HDLLEVINLE DNKIAELREI EYIKNLPILR VLNLLENPIQ EKSEYWFFVI FMLLRLTELD
361QKKIKVEEKV SAVNKYDPPP EVVAAQDHLT HVVNSVMQPQ RIFDSTLPSL DAPYPMLILA
421GPEACGKREL AHRLCRQFST YFRYGACHTT RPPYFGEGDR VDYHFISQDV FDEMVNMGKF
481ILTFSYGNHK YGLNRDTVEG IARDGLASCI HMEIEGVRSL KYSYFEPRYI LVVPMNKEKY
541EGYLRRKGLF SRAEIEFAVS RVDLYIKINQ NFPGYFDEVI NADDLDVAYQ KLSQLIREYL
601GLTEELAKSL ATTADVKTSH LKPEAHPTKY ISSNMGDFLH STDRNYLIKF WAKLSAKKTP
661AERDSIHRQH EAARQALMGR IRPDHTLLFQ RGPVPAPLTS GLHYYTTLEE LWKSFDLCED
721YFKPPFGPYP EKSGKDSLVS MKCSLFRFCP WSKELPFQPP EGSISSHLGS GASDSETEET
781RKALPIQSFS HEKESHQHRQ HSVPVISRPG SNVKPTLPPI PQGRR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

825 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.