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LRFN5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRFN5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRFN5 antibodies →
$504.00each
Catalog Number: LS-G106211-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRFN5 (NM_152447), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf146 | FIGLER8 | LRFN5 | SALM5
Origin Species
Human
SwissProt
Q96NI6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKILFYLFL IGIAVKAQIC PKRCVCQILS PNLATLCAKK GLLFVPPNID RRTVELRLAD
61NFVTNIKRKD FANMTSLVDL TLSRNTISFI TPHAFADLRN LRALHLNSNR LTKITNDMFS
121GLSNLHHLIL NNNQLTLISS TAFDDVFALE ELDLSYNNLE TIPWDAVEKM VSLHTLSLDH
181NMIDNIPKGT FSHLHKMTRL DVTSNKLQKL PPDPLFQRAQ VLATSGIISP STFALSFGGN
241PLHCNCELLW LRRLSREDDL ETCASPPLLT GRYFWSIPEE EFLCEPPLIT RHTHEMRVLE
301GQRATLRCKA RGDPEPAIHW ISPEGKLISN ATRSLVYDNG TLDILITTVK DTGAFTCIAS
361NPAGEATQIV DLHIIKLPHL LNSTNHIHEP DPGSSDISTS TKSGSNTSSS NGDTKLSQDK
421IVVAEATSST ALLKFNFQRN IPGIRMFQIQ YNGTYDDTLV YRMIPPTSKT FLVNNLAAGT
481MYDLCVLAIY DDGITSLTAT RVVGCIQFTT EQDYVRCHFM QSQFLGGTMI IIIGGIIVAS
541VLVFIIILMI RYKVCNNNGQ HKVTKVSNVY SQTNGAQIQG CSVTLPQSVS KQAVGHEENA
601QCCKATSDNV IQSSETCSSQ DSSTTTSALP PSWTSSTSVS QKQKRKTGTK PSTEPQNEAV
661TNVESQNTNR NNSTALQLAS RPPDSVTEGP TSKRAHIKPN ALLTNVDQIV QETQRLELI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

719 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.