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LRFN4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRFN4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110100-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRFN4 (NM_024036), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FIGLER6 | SALM3 | LRFN4 | SALM3.
Origin Species
Human
NCBI GenBank
NM_024036
SwissProt
Q6PJG9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPPLLLLLL ASGAAACPLP CVCQNLSESL STLCAHRGLL FVPPNVDRRT VELRLADNFI
61QALGPPDFRN MTGLVDLTLS RNAITRIGAR AFGDLESLRS LHLDGNRLVE LGTGSLRGPV
121NLQHLILSGN QLGRIAPGAF DDFLESLEDL DLSYNNLRQV PWAGIGAMPA LHTLNLDHNL
181IDALPPGAFA QLGQLSRLDL TSNRLATLAP DPLFSRGRDA EASPAPLVLS FSGNPLHCNC
241ELLWLRRLAR PDDLETCASP PGLAGRYFWA VPEGEFSCEP PLIARHTQRL WVLEGQRATL
301RCRALGDPAP TMHWVGPDDR LVGNSSRARA FPNGTLEIGV TGAGDAGGYT CIATNPAGEA
361TARVELRVLA LPHGGNSSAE GGRPGPSDIA ASARTAAEGE GTLESEPAVQ VTEVTATSGL
421VSWGPGRPAD PVWMFQIQYN SSEDETLIYR IVPASSHHFL LKHLVPGADY DLCLLALSPA
481AGPSDLTATR LLGCAHFSTL PASPLCHALQ AHVLGGTLTV AVGGVLVAAL LVFTVALLVR
541GRGAGNGRLP LKLSHVQSQT NGGPSPTPKA HPPRSPPPRP QRSCSLDLGD AGCYGYARRL
601GGAWARRSHS VHGGLLGAGC RGVGGSAERL EESVV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

635 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.