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LRFN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRFN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LRFN1 antibodies →
$725.00each
Catalog Number: LS-G110916-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRFN1 (NM_020862), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1484 | LRFN1 | SALM2
Origin Species
Human
NCBI GenBank
AB040917
SwissProt
Q9P244

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPGPFSSAL LSPPPAALPF LLLLWAGASR GQPCPGRCIC QNVAPTLTML CAKTGLLFVP
61PAIDRRVVEL RLTDNFIAAV RRRDFANMTS LVHLTLSRNT IGQVAAGAFA DLRALRALHL
121DSNRLAEVRG DQLRGLGNLR HLILGNNQIR RVESAAFDAF LSTVEDLDLS YNNLEALPWE
181AVGQMVNLNT LTLDHNLIDH IAEGTFVQLH KLVRLDMTSN RLHKLPPDGL FLRSQGTGPK
241PPTPLTVSFG GNPLHCNCEL LWLRRLTRED DLETCATPEH LTDRYFWSIP EEEFLCEPPL
301ITRQAGGRAL VVEGQAVSLR CRAVGDPEPV VHWVAPDGRL LGNSSRTRVR GDGTLDVTIT
361TLRDSGTFTC IASNAAGEAT APVEVCVVPL PLMAPPPAAP PPLTEPGSSD IATPGRPGAN
421DSAAERRLVA AELTSNSVLI RWPAQRPVPG IRMYQVQYNS SVDDSLVYRM IPSTSQTFLV
481NDLAAGRAYD LCVLAVYDDG ATALPATRVV GCVQFTTAGD PAPCRPLRAH FLGGTMIIAI
541GGVIVASVLV FIVLLMIRYK VYGDGDSRRV KGSRSLPRVS HVCSQTNGAG TGAAQAPALP
601AQDHYEALRE VESQAAPAVA VEAKAMEAET ASAEPEVVLG RSLGGSATSL CLLPSEETSG
661EESRAAVGPR RSRSGALEPP TSAPPTLALV PGGAAARPRP QQRYSFDGDY GALFQSHSYP
721RRARRTKRHR STPHLDGAGG GAAGEDGDLG LGSARACLAF TSTEWMLEST V
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

771 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.