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LRCH4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRCH4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G118037-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRCH4 (NM_002319), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Leucine-rich neuronal protein | Leucine rich repeat neuronal 4 | LRCH4 | PP14183 | LRN
Origin Species
Human
NCBI GenBank
NM_002319
SwissProt
O75427

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAVAAPLA AGGEEAAATT SVPGSPGLPG RRSAERALEE AVATGTLNLS NRRLKHFPRG
61AARSYDLSDI TQADLSRNRF PEVPEAACQL VSLEGLSLYH NCLRCLNPAL GNLTALTYLN
121LSRNQLSLLP PYICQLPLRV LIVSNNKLGA LPPDIGTLGS LRQLDVSSNE LQSLPSELCG
181LSSLRDLNVR RNQLSTLPEE LGDLPLVRLD FSCNRVSRIP VSFCRLRHLQ VILLDSNPLQ
241SPPAQVCLKG KLHIFKYLST EAGQRGSALG DLAPSRPPSF SPCPAEDLFP GHRYDGGLDS
301GFHSVDSGSK RWSGNESTDE FSELSFRISE LAREPRGPRE RKEDGSADGD PVQIDFIDSH
361VPGEDEERGT VEEQRPPELS PGAGDRERAP SSRREEPAGE ERRRPDTLQL WQERERRQQQ
421QSGAWGAPRK DSLLKPGLRA VVGGAAAVST QAMHNGSPKS SASQAGAAAG QGAPAPAPAS
481QEPLPIAGPA TAPAPRPLGS IQRPNSFLFR SSSQSGSGPS SPDSVLRPRR YPQVPDEKDL
541MTQLRQVLES RLQRPLPEDL AEALASGVIL CQLANQLRPR SVPFIHVPSP AVPKLSALKA
601RKNVESFLEA CRKMGVPEAD LCSPSDLLQG TARGLRTALE AVKRVGGKAL PPLWPPSGLG
661GFVVFYVVLM LLLYVTYTRL LGSTRTRPLE QKLISEEDLA ANDILDYKDD DDKV

714 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.