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LRCH3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LRCH3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G108645-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRCH3 (NM_032773), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LRCH3
Origin Species
Human
NCBI GenBank
NM_032773
SwissProt
Q96II8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAGLVAVA AAAEYSGTVA SGGNLPGVHC GPSSGAGPGF GPGSWSRSLD RALEEAAVTG
61VLSLSGRKLR EFPRGAANHD LTDTTRADLS RNRLSEIPIE ACHFVSLENL NLYQNCIRYI
121PEAILNLQAL TFLNISRNQL STLPVHLCNL PLKVLIASNN KLVSLPEEIG HLRHLMELDV
181SCNEIQTIPS QIGNLEALRD LNVRRNHLVH LPEELAELPL IRLDFSCNKI TTIPVCYRNL
241RHLQTITLDN NPLQSPPAQI CIKGKVHIFK YLNIQACKIA PDLPDYDRRP LGFGSCHEEL
301YSSRPYGALD SGFNSVDSGD KRWSGNEPTD EFSDLPLRVA EITKEQRLRR ESQYQENRGS
361LVVTNGGVEH DLDQIDYIDS CTAEEEEAEV RQPKGPDPDS LSSQFMAYIE QRRISHEGSP
421VKPVAIREFQ KTEDMRRYLH QNRVPAEPSS LLSLSASHNQ LSHTDLELHQ RREQLVERTR
481REAQLAALQY EEEKIRTKQI QRDAVLDFVK QKASQSPQKQ HPLLDGVDGE CPFPSRRSQH
541TDDSALCMSL SGLNQVGCAA TLPHSSAFTP LKSDDRPNAL LSSPATETVH HSPAYSFPAA
601IQRNQPQRPE SFLFRAGVRA ETNKGHASPL PPSAAPTTDS TDSITGQNSR QREEELELID
661QLRKHIEYRL KVSLPCDLGA ALTDGVVLCH LANHVRPRSV PSIHVPSPAV VS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

712 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.