Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LPO / Lactoperoxidase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115501-100
Catalog Number: LS-G115501
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Lactoperoxidase(LPO) (NM_006151), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Lactoperoxidase | Salivary peroxidase | LPO | SPO | SAPX
- Origin Species
- Human
- NCBI GenBank
- U39573
- SwissProt
- P22079
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.29 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRVLLHLPAL LASLILLQAA ASTTRAQTTR TSAISDTVSQ AKVQVNKAFL DSRTRLKTAM61SSETPTSRQL SEYLKHAKGR TRTAIRNGQV WEESLKRLRQ KASLTNVTDP SLDLTSLSLE121VGCGAPAPVV RCDPCSPYRT ITGDCNNRRK PALGAANRAL ARWLPAEYED GLSLPFGWTP181GKTRNGFPLP LAREVSNKIV GYLNEEGVLD QNRSLLFMQW GQIVDHDLDF APDTELGSSE241YSKAQCDEYC IQGDNCFPIM FPPNDPKAGT QGKCMPFFRA GFVCPTPPYK SLAREQINAL301TSFLDASFVY SSEPSLASRL RNLSSPLGLM AVNQEVSDHG LPYLPYDSKK PSPCEFINTT361ARVPCFLAGD SRASEHILLA TSHTLFLREH NRLARELKRL NPQWDGEKLY QEARKILGAF421VQIITFRDYL PILLGDHMQK WIPPYQGYSE SVDPRISNVF TFAFRFGHLE VPSSMFRLDE481NYQPWGPEPE LPLHTLFFNT WRMVKDGGID PLVRGLLAKK SKLMKQNKMM TGELRNKLFQ541PTHRIHGFDL AAINTQRCRD HGQPGYNSWR AFCDLSQPQT LEELNTVLKS KMLAKKLLGL601YGTPDNIDIW IGAIAEPLVE RGRVGPLLAC LLGKQFQQIR DGDRFWWENP GVFTNEQKDS661LQKMSFSRLV CDNTRITKVP RDPFWANSYP YDFVDCSAID KLDLSPWASV KNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
712 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.