Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LPIN1 / Lipin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106615-100
Catalog Number: LS-G106615
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Lipin 1(LPIN1) (NM_145693), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Lipin-1 | Lipin 1 | PAP1 | KIAA0188 | LPIN1
- Origin Species
- Human
- NCBI GenBank
- D80010
- SwissProt
- Q14693
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNYVGQLAGQ VFVTVKELYK GLNPATLSGC IDIIVIRQPN GNLQCSPFHV RFGKMGVLRS61REKVVDIEIN GESVDLHMKL GDNGEAFFVQ ETDNDQEVIP MHLATSPILS EGASRMECQL121KRGSVDRMRG LDPSTPAQVI APSETPSSSS VVKKRRKRRR KSQLDSLKRD DNMNTSEDED181MFPIEMSSDE AMELLESSRT LPNDIPPFQD DIPEENLSLA VIYPQSASYP NSDREWSPTP241SPSGSRPSTP KSDSELVSKS TERTGQKNPE MLWLWGELPQ AAKSSSPHKM KESSPLSSRK301ICDKSHFQAI HSESSDTFSD QSPTLVGGAL LDQNKPQTEM QFVNEEDLET LGAAAPLLPM361IEELKPPSAS VVQTANKTDS PSRKRDKRSR HLGADGVYLD DLTDMDPEVA ALYFPKNGDP421SGLAKHASDN GARSANQSPQ SVGSSGVDSG VESTSDGLRD LPSIAISLCG GLSDHREITK481DAFLEQAVSY QQFVDNPAII DDPNLVVKIG SKYYNWTTAA PLLLAMQAFQ KPLPKATVES541IMRDKMPKKG GRWWFSWRGR NTTIKEESKP EQCLAGKAHS TGEQPPQLSL ATRVKHESSS601SDEERAAAKP SNAGHLPLLP NVSYKKTLRL TSEQLKSLKL KNGPNDVVFS VTTQYQGTCR661CEGTIYLWNW DDKVIISDID GTITRSDTLG HILPTLGKDW THQGIAKLYH KVSQNGYKFL721YCSARAIGMA DMTRGYLHWV NERGTVLPQG PLLLSPSSLF SALHREVIEK KPEKFKVQCL781TDIKNLFFPN TEPFYAAFGN RPADVYSYKQ VGVSLNRIFT VNPKGELVQE HAKTNISSYV841RLCEVVDHVF PLLKRSHSSD FPCSDTFSNF TFWREPLPPF ENQDIHSASATRTRPLEQKLISEEDLAANDILDYKDDDDKV
890 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.