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LPCAT4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LPCAT4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102398-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AGPAT7(LPCAT4) (NM_153613), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
1-AGPAT 7 | Acyltransferase like 3 | Acyltransferase-like 3 | 1-AGP acyltransferase 7 | AGPAT7 | LPCAT4 | LPEAT2 | PLSC domain containing protein | AYTL3 | LPAAT-eta
Origin Species
Human
NCBI GenBank
NM_153613
SwissProt
Q643R3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSQGSPGDWA PLDPTPGPPA SPNPFVHELH LSRLQRVKFC LLGALLAPIR VLLAFIVLFL
61LWPFAWLQVA GLSEEQLQEP ITGWRKTVCH NGVLGLSRLL FFLLGFLRIR VRGQRASRLQ
121APVLVAAPHS TFFDPIVLLP CDLPKVVSRA ENLSVPVIGA LLRFNQAILV SRHDPASRRR
181VVEEVRRRAT SGGKWPQVLF FPEGTCSNKK ALLKFKPGAF IAGVPVQPVL IRYPNSLDTT
241SWAWRGPGVL KVLWLTASQP CSIVDVEFLP VYHPSPEESR DPTLYANNVQ RVMAQALGIP
301ATECEFVGSL PVIVVGRLKV ALEPQLWELG KVLRKAGLSA GYVDAGAEPG RSRMISQEEF
361ARQLQLSDPQ TVAGAFGYFQ QDTKGLVDFR DVALALAALD GGRSLEELTR LAFELFAEEQ
421AEGPNRLLYK DGFSTILHLL LGSPHPAATA LHAELCQAGS SQGLSLCQFQ NFSLHDPLYG
481KLFSTYLRPP HTSRGTSQTP NASSPGNPTA LANGTVQAPK QKGD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

524 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.