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LPCAT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LPCAT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LPCAT2 antibodies →
$504.00each
Catalog Number: LS-G101506-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Acyltransferase like 1(LPCAT2) (NM_017839), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AYTL1 | Acyltransferase like 1 | Acyltransferase-like 1 | LPCAT-2 | LPCAT2 | Lyso-PAF acetyltransferase | LysoPC acyltransferase 2 | LPC acyltransferase 2 | LysoPAFAT
Origin Species
Human
NCBI GenBank
NM_017839
SwissProt
Q7L5N7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60 kda
Protein Type
Lysate
Amino Acid Sequence
1MSRCAQAAEV AATVPGAGVG NVGLRPPMVP RQASFFPPPV PNPFVQQTQI GSARRVQIVL
61LGIILLPIRV LLVALILLLA WPFAAISTVC CPEKLTHPIT GWRRKITQTA LKFLGRAMFF
121SMGFIVAVKG KIASPLEAPV FVAAPHSTFF DGIACVVAGL PSMVSRNENA QVPLIGRLLR
181AVQPVLVSRV DPDSRKNTIN EIIKRTTSGG EWPQILVFPE GTCTNRSCLI TFKPGAFIPG
241VPVQPVLLRY PNKLDTVTWT WQGYTFIQLC MLTFCQLFTK VEVEFMPVQV PNDEEKNDPV
301LFANKVRNLM AEALGIPVTD HTYEDCRLMI SAGQLTLPME AGLVEFTKIS RKLKLDWDGV
361RKHLDEYASI ASSSKGGRIG IEEFAKYLKL PVSDVLRQLF ALFDRNHDGS IDFREYVIGL
421AVLCNPSNTE EIIQVAFKLF DVDEDGYITE EEFSTILQAS LGVPDLDVSG LFKEIAQGDS
481ISYEEFKSFA LKHPEYAKIF TTYLDLQTCH VFSLPKEVQT TPSTASNKVS PEKHEESTSD
541KKDDTRTRPL EQKLISEEDL AANDILDYKD DDDKV

575 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.