Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LPAR4 / GPR23 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100514-100
Catalog Number: LS-G100514
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human P2Y9(LPAR4) (NM_005296), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G-protein coupled receptor 23 | LPA-4 | p2RY9 | p2Y5-LIKE | LPAR4 | p2Y purinoceptor 9 | p2Y5-like receptor | p2Y9 | G protein-coupled receptor 23 | GPR23 | LPA receptor 4 | LPA4 | Purinergic receptor 9
- Origin Species
- Human
- NCBI GenBank
- NM_005296
- SwissProt
- Q99677
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGDRRFIDFQ FQDSNSSLRP RLGNATANNT CIVDDSFKYN LNGAVYSVVF ILGLITNSVS61LFVFCFRMKM RSETAIFITN LAVSDLLFVC TLPFKIFYNF NRHWPFGDTL CKISGTAFLT121NIYGSMLFLT CISVDRFLAI VYPFRSRTIR TRRNSAIVCA GVWILVLSGG ISASLFSTTN181VNNATTTCFE GFSKRVWKTY LSKITIFIEV VGFIIPLILN VSCSSVVLRT LRKPATLSQI241GTNKKKVLKM ITVHMAVFVV CFVPYNSVLF LYALVRSQAI TNCFLERFAK IMYPITLCLA301TLNCCFDPFI YYFTLESFQK SFYINAHIRM ESLFKTETPL TTKPSLPAIQ EEVSDQTTNN361GGELMLESTFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
370 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.