Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LOXL4 / LOXC Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108970-100
Catalog Number: LS-G108970
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LOXL4 (NM_032211), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LOXC | Lysyl oxidase-like 4 | Lysyl oxidase-like protein 4 | LOXL4 | Lysyl oxidase related C | Lysyl oxidase homolog 4
- Origin Species
- Human
- NCBI GenBank
- NM_032211
- SwissProt
- Q96JB6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 81.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MARSPPATLF LFLLLLGQPP PSRPQSLGTT KLRLVGPESK PEEGRLEVLH QGQWGTVCDD61NFAIQEATVA CRQLGFEAAL TWAHSAKYGQ GEGPIWLDNV QCVGTESSLD QCGSNGWGVS121DCSHSEDVGV ICHPRRHRGY LSETVSNALG PQGRRLEEVR LKPILASAKQ HSPVTEGAVE181VKYEGHWRQV CDQGWTMNNS RVVCGMLGFP SEVPVDSHYY RKVWDLKMRD PKSRLKSLTN241KNSFWIHQVT CLGTEPHMAN CQVQVAPARG KLRPACPGGM HAVVSCVAGP HFRPPKTKPQ301RKGSWAEEPR VRLRSGAQVG EGRVEVLMNR QWGTVCDHRW NLISASVVCR QLGFGSAREA361LFGARLGQGL GPIHLSEVRC RGYERTLSDC PALEGSQNGC QHENAAAVRC NVPNMGFQNQ421VRLAGGRIPE EGLLEVQVEV NGVPRWGSVC SENWGLTEAM VACRQLGLGF AIHAYKETWF481WSGTPRAQEV VMSGVRCSGT ELALQQCQRH GPVHCSHGGG RFLAGVSCMD SAPDLVMNAQ541LVQETAYLED RPLSQLYCAH EENCLSKSAD HMDWPYGYRR LLRFSTQIYN LGRTDFRPKT601GRDSWVWHQC HRHYHSIEVF THYDLLTLNG SKVAEGHKAS FCLEDTNCPT GLQRRYACAN661FGEQGVTVGC WDTYRHDIDC QWVDITDVGP GNYIFQVIVN PHYEVAESDF SNNMLQCRCK721YDGHRVWLHN CHTGNSYPAN AELSLEQEQR LRNNLITRTRPLEQKLISEEDLAANDILDYKDDDDKV
756 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.