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LOXHD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LOXHD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G126739-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LOXHD1 (NM_001145473), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_144612

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTVWTGDVVG GGTDSNIFMT LYGINGSTEE MQLDKKKARF EREQNDTFIM EILDIAPFTK
61MRIRIDGLGS RPEWFLERIL LKNMNTGDLT MFYYGDWLSQ RKGKKTLVCE MCAVIDEEEM
121MEWTSYTVAV KTSDILGAGT DANVFIIIFG ENGDSGTLAL KQSANWNKFE RNNTDTFNFP
181DMLSLGHLCK LRVWHDNKGI FPGWHLSYVD VKDNSRDETF HFQCDCWLSK SEGDGQTVRD
241FACANNKICD ELEETTYEIV IETGNGGETR ENVWLILEGR KNRSKEFLME NSSRQRAFRK
301GTTDTFEFDS IYLGDIASLC VGHLAREDRF IPKRELAWHV KTITITEMEY GNVYFFNCDC
361LIPLKRKRKY FKVFEVTKTT ESFASKVQSL VPVKYEVIVT TGYEPGAGTD ANVFVTIFGA
421NGDTGKRELK QKMRNLFERG STDRFFLETL ELGELRKVRL EHDSSGYCSG WLVEKVEVTN
481TSTGVATIFN CGRWLDKKRG DGLTWRDLFP SV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

512 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.