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LONRF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LONRF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G99291-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LONRF3 (NM_001031855), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RING finger protein 127 | RNF127 | LONRF3
NCBI GenBank
NM_024778
SwissProt
Q496Y0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESVRIEQML SLPAEVSSDN LESAERGASA AQVDMGPHPK VAAEGPAPLP TREPEQEQSP
61GTSTPESKVL LTQADALASR GRIREALEVY RQLSERQQLV AEQLEQLVRC LAEKVPQGEA
121LAPAPPDEGS TASGTVAAEE TGAAAAAAAT EVWDGFKCRK CHGFLSDPVS LSCGHTFCKL
181CLERGRAADR RCALCGVKLS ALMVATGRAR GARRAGQQPP PPLRVNVVLS GLLGKLFPGP
241ARASQLRHEG NRLYRERQVE AALLKYNEAV KLAPNDHLLY SNRSQIYFTL ESHENALHDA
301EIACKLRPMG FKAHFRKAQA LATLGKVEEA LREFLYCVSL DGKNKRARCE AQRDNLELPH
361CSSQEEAAAR GDGSSLMDPA KVKGDGQQHH MKDQEEEEEK WDATSPKAAS SKTGKCQEKK
421RKHCQIESQE ETGMPNKASK QDPPTDQGDK PALSLPLASF DASDLECALC MRLFYEPVTT
481PCGHTFCLKC LERCLDHNAK CPLCKDGLSQ CLASRKYSKN VIMEELIAKF LPEELKERRK
541LYEEEMEELS NLNKNVPIFV CTMAYPTVPC PLHIFEPCYR LMIRRCIETG TRQFGMCLGD
601PVKGFAEYGC ILEIRNVQFL ADGRSVVDSI GKRRFRVLHQ SQRDGYNTAD IEYIEDQKVQ
661GEDCAELMGL HNCVYQQASL WFHSLKLSLK NRILNHFGPM PEKDADPQMN PNGPAWCWWM
721LAVLPLESRA QLPFLAMRSL KDRLNGIRRV LAFISRNQN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

759 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.