Human LONRF3 Protein (Recombinant GST, N-terminus + His, C-terminus)
Catalog Number: LS-G145229-50
50 µg / $428.00
Expression System Escherichia coli (Prokaryotic)
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LONRF3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99291-100
Catalog Number: LS-G99291
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LONRF3 (NM_001031855), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RING finger protein 127 | RNF127 | LONRF3
- NCBI GenBank
- NM_024778
- SwissProt
- Q496Y0
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 84.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MESVRIEQML SLPAEVSSDN LESAERGASA AQVDMGPHPK VAAEGPAPLP TREPEQEQSP61GTSTPESKVL LTQADALASR GRIREALEVY RQLSERQQLV AEQLEQLVRC LAEKVPQGEA121LAPAPPDEGS TASGTVAAEE TGAAAAAAAT EVWDGFKCRK CHGFLSDPVS LSCGHTFCKL181CLERGRAADR RCALCGVKLS ALMVATGRAR GARRAGQQPP PPLRVNVVLS GLLGKLFPGP241ARASQLRHEG NRLYRERQVE AALLKYNEAV KLAPNDHLLY SNRSQIYFTL ESHENALHDA301EIACKLRPMG FKAHFRKAQA LATLGKVEEA LREFLYCVSL DGKNKRARCE AQRDNLELPH361CSSQEEAAAR GDGSSLMDPA KVKGDGQQHH MKDQEEEEEK WDATSPKAAS SKTGKCQEKK421RKHCQIESQE ETGMPNKASK QDPPTDQGDK PALSLPLASF DASDLECALC MRLFYEPVTT481PCGHTFCLKC LERCLDHNAK CPLCKDGLSQ CLASRKYSKN VIMEELIAKF LPEELKERRK541LYEEEMEELS NLNKNVPIFV CTMAYPTVPC PLHIFEPCYR LMIRRCIETG TRQFGMCLGD601PVKGFAEYGC ILEIRNVQFL ADGRSVVDSI GKRRFRVLHQ SQRDGYNTAD IEYIEDQKVQ661GEDCAELMGL HNCVYQQASL WFHSLKLSLK NRILNHFGPM PEKDADPQMN PNGPAWCWWM721LAVLPLESRA QLPFLAMRSL KDRLNGIRRV LAFISRNQNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
759 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.
