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LONRF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LONRF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106416-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LONRF1 (NM_152271), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RNF191 | LONRF1 | RING finger protein 191
Origin Species
Human
NCBI GenBank
NM_152271
SwissProt
Q17RB8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSPAVARTS PGGSREMAPA PQGRGRFWEV GGGSGHRLER AAAESERWEL LLRRGELLAL
61GGHLKGALEA FAAALRRGAP ARPECLGALV DCLVFNYRLR HGLGWSAAPV AGADGGAGGL
121LRCLGCRGFL SEPVTVPCGH SYCRRCLRRE LRARCRLCRD RLPPATASAT DAEGTAPRPP
181PLAAAIAASD FRTSVVLNHL AEKWFPGQRE RARAAGRLGE LLHQGRYREA LAAACEALRA
241EPSDLIVKIY RAESYAGLQE FKAAIEDLNA VLFQLPDWPE VYFRKGKVLC DAGFLGDALQ
301LFLQCLALDE DFAPAKLQVQ KILCDLLLPE NLKEGLKESS WSSLPCTKNR PFDFHSVMEE
361SQSLNEPSPK QSEEIPEVTS EPVKGSLNRA QSAQSINSTE MPAREDCLKR VSSEPVLSVQ
421EKGVLLKRKL SLLEQDVIVN EDGRNKLKKQ GETPNEVCMF SLAYGDIPEE LIDVSDFECS
481LCMRLFFEPV TTPCGHSFCK NCLERCLDHA PYCPLCKESL KEYLADRRYC VTQLLEELIV
541KYLPDELSER KKIYDEETAE LSHLTKNVPI FVCTMAYPTV PCPLHVFEPR YRLMIRRSIQ
601TGTKQFGMCV SDTQNSFADY GCMLQIRNVH FLPDGRSVVD TVGGKRFRVL KRGMKDGYCT
661ADIEYLEDVK VENEDEIKNL RELHDLVYSQ ACSWFQNLRD RFRSQILQHF GSMPEREENL
721QAAPNGPAWC WWLLAVLPVD PRYQLSVLSM KSLKERLTKI QHILTYFSRD QSK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

773 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.