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LOC81691 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LOC81691 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LOC81691 antibodies →
$504.00each
Catalog Number: LS-G101983-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LOC81691 (NM_030941), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Exonuclease NEF-sp | LOC81691
Origin Species
Human
NCBI GenBank
NM_030941
SwissProt
Q96IC2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPEREGTER HPRKVRESRQ APNKLVGAAE AMKAGWDLEE SQPEAKKARL STILFTDNCE
61VTHDQLCELL KYAVLGKSNV PKPSWCQLFH QNHLNNVVVF VLQGMSQLHF YRFYLEFGCL
121RKAFRHKFRL PPPSSDFLAD VVGLQTEQRA GDLPKTMEGP LPSNAKAAIN LQDDPIIQKY
181GSKKVGLTRC LLTKEEMRTF HFPLQGFPDC ENFLLTKCNG SIADNSPLFG LDCEMCLTSK
241GRELTRISLV AEGGCCVMDE LVKPENKILD YLTSFSGITK KILNPVTTKL KDVQRQLKAL
301LPPDAVLVGH SLDLDLRALK MIHPYVIDTS LLYVREQGRR FKLKFLAKVI LGKDIQCPDR
361LGHDATEDAR TILELARYFL KHGPKKIAEL NLEALANHQE IQAAGQEPKN TAEVLQHPNT
421SVLECLDSVG QKLLFLTRET DAGELPSSRN CQTIKCLSNK EVLEQARVEI PLFPFSIVQF
481SFKAFSPVLT EEMNKRMRIK WTEISTVYAG PFSKNCNLRA LKRLFKSFGP VQSMTFVLET
541RQPHLCIQYE VLEAAQLAIE SLDGILVDGI CIKVQRPVTE LTLDCDTLVN ELEGDSENQG
601SIYLSGVSET FKEQLLQEPR LFLGLEAVIL PKDLKSGKQK KYCFLKFKSF GSAQQALNIL
661TGKDWKLKGR HALTPRHLHA WLRGLPPEST RLPGLRVVPP PFEQEALQTL KLDHPKIAAW
721RWSRKIGKLY NSLCPGTLCL ILLPGTKSTH GSLSGLGLMG IKEEEESAGP GLCS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

774 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.