LSBio
LNX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LNX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LNX2 antibodies →
$504.00each
Catalog Number: LS-G105814-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LNX2 (NM_153371), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ligand of Numb protein X 2 | Ligand of numb-protein X 2 | Numb-binding protein 2 | PDZRN1 | LNX2
Origin Species
Human
NCBI GenBank
NM_153371
SwissProt
Q8N448

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGTTSDEMVS VEQTSSSSLN PLCFECGQQH WTRENHLYNY QNEVDDDLVC HICLQPLLQP
61LDTPCGHTFC YKCLRNFLQE KDFCPLDRKR LHFKLCKKSS ILVHKLLDKL LVLCPFSSVC
121KDVMQRCDLE AHLKNRCPGA SHRRVALERR KTSRTQAEIE NENGPTLLDP AGTLSPEADC
181LGTGAVPVER HLTSASLSTW SEEPGLDNPA FEESAGADTT QQPLSLPEGE ITTIEIHRSN
241PYIQLGISIV GGNETPLINI VIQEVYRDGV IARDGRLLAG DQILQVNNYN ISNVSHNYAR
301AVLSQPCNTL HLTVLRERRF GNRAHNHSDS NSPREEIFQV ALHKRDSGEQ LGIKLVRRTD
361EPGVFILDLL EGGLAAQDGR LSSNDRVLAI NGHDLKYGTP ELAAQIIQAS GERVNLTIAR
421PGKPQPGNTI REAGNHSSSS QHHTPPPYYS RPSSHKDLTQ CVTCQEKHIT VKKEPHESLG
481MTVAGGRGSK SGELPIFVTS VPPHGCLARD GRIKRGDVLL NINGIDLTNL SHSEAVAMLK
541ASAASPAVAL KALEVQIVEE ATQNAEEQPS TFSENEYDAS WSPSWVMWLG LPSTLHSCHD
601IVLRRSYLGS WGFSIVGGYE ENHTNQPFFI KTIVLGTPAY YDGRLKCGDM IVAVNGLSTV
661GMSHSALVPM LKEQRNKVTL TVICWPGSLV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

690 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.