Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LNX1 / LNX Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102062-100
Catalog Number: LS-G102062
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LNX1 (NM_032622), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Ligand of numb-protein X | LNX | Ligand of Numb-protein X 1 | LNX1 | Numb-binding protein 1 | PDZRN2
- Origin Species
- Human
- NCBI GenBank
- NM_032622
- SwissProt
- Q8TBB1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKALLLLVLP WLSPANYIDN VGNLHFLYSE LCKGASHYGL TKDRKRRSQD GCPDGCASLT61ATAPSPEVSA AATISLMTDE PGLDNPAYVS SAEDGQPAIS PVDSGRSNRT RARPFERSTI121RSRSFKKINR ALSVLRRTKS GSAVANHADQ GRENSENTTA PEVFPRLYHL IPDGEITSIK181INRVDPSESL SIRLVGGSET PLVHIIIQHI YRDGVIARDG RLLPGDIILK VNGMDISNVP241HNYAVRLLRQ PCQVLWLTVM REQKFRSRNN GQAPDAYRPR DDSFHVILNK SSPEEQLGIK301LVRKVDEPGV FIFNVLDGGV AYRHGQLEEN DRVLAINGHD LRYGSPESAA HLIQASERRV361HLVVSRQVRQ RSPDIFQEAG WNSNGSWSPG PGERSNTPKP LHPTITCHEK VVNIQKDPGE421SLGMTVAGGA SHREWDLPIY VISVEPGGVI SRDGRIKTGD ILLNVDGVEL TEVSRSEAVA481LLKRTSSSIV LKALEVKEYE PQEDCSSPAA LDSNHNMAPP SDWSPSWVMW LELPRCLYNC541KDIVLRRNTA GSLGFCIVGG YEEYNGNKPF FIKSIVEGTP AYNDGRIRCG DILLAVNGRS601TSGMIHACLA RLLKELKGRI TLTIVSWPGT FLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
632 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.