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LMX1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LMX1B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G118054-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LMX1B (NM_002316), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LMX1.2 | LMX1B | NPS1 | LIM/homeobox protein 1.2 | LMX-1.2 | LIM/homeobox protein LMX1B
Origin Species
Human
NCBI GenBank
NM_002316

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
41.71 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLDGIKMEEH ALRPGPATLG VLLGSDCPHP AVCEGCQRPI SDRFLMRVNE SSWHEECLQC
61AACQQALTTS CYFRDRKLYC KQDYQQLFAA KCSGCMEKIA PTEFVMRALE CVYHLGCFCC
121CVCERQLRKG DEFVLKEGQL LCKGDYEKEK DLLSSVSPDE SDSVKSEDED GDMKPAKGQG
181SQSKGSGDDG KDPRRPKRPR TILTTQQRRA FKASFEVSSK PCRKVRETLA AETGLSVRVV
241QVWFQNQRAK MKKLARRHQQ QQEQQNSQRL GQEVLSSRME GMMASYTPLA PPQQQIVAME
301QSPYGSSDPF QQGLTPPQMP GNDSIFHDID SDTSLTSLSD CFLGSSDVGS LQARVGNPID
361RLYSMQSSYF AS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

372 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.