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LMOD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LMOD3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103764-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Leiomodin 3(LMOD3) (NM_198271), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Leiomodin 3 (fetal) | Leiomodin-3 | LMOD3 | Leiomodin | fetal form
Origin Species
Human
NCBI GenBank
AA448855

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSEHSRNSDQ EELLDEEINE DEILANLSAE ELKELQSEME VMAPDPSLPV GMIQKDQTDK
61PPTGNFNHKS LVDYMYWEKA SRRMLEEERV PVTFVKSEEK TQEEHEEIEK RNKNMAQYLK
121EKLNNEIVAN KRESKGSSNI QETDEEDEEE EDDDDDDEGE DDGEESEETN REEEGKAKEQ
181IRNCENNCQQ VTDKAFKEQR DRPEAQEQSE KKISKLDPKK LALDTSFLKV STRPSGNQTD
241LDGSLRRVRK NDPDMKELNL NNIENIPKEM LLDFVNAMKK NKHIKTFSLA NVGADENVAF
301ALANMLRENR SITTLNIESN FITGKGIVAI MRCLQFNETL TELRFHNQRH MLGHHAEMEI
361ARLLKANNTL LKMGYHFELP GPRMVVTNLL TRNQDKQRQK RQEEQKQQQL KEQKKLIAML
421ENGLGLPPGM WELLGGPKPD SRMQEFFQPP PPRPPNPQNV PFSQRSEMMK KPSQAPKYRT
481DPDSFRVVKL KRIQRKSRMP EAREPPEKTN LKDVIKTLKP VPRNRPPPLV EITPRDQLLN
541DIRHSSVAYL KPVQLPKELA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

560 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.