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LMO7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LMO7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LMO7 antibodies →
$725.00each
Catalog Number: LS-G116018-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LMO7 (NM_005358), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FBX20 | FBXO20 | F-box only protein 20 | LIM domain only 7 protein | LIM domain only protein 7 | LMO7 | LOMP | LIM domain only 7 | F-box protein Fbx20 | KIAA0858 | LIM domain 7 | LMO-7
Origin Species
Human
NCBI GenBank
NM_005358
SwissProt
Q8WWI1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
153.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKKIRICHIF TFYSWMSYDV LFQRTELGAL EIWRQLICAH VCICVGWLYL RDRVCSKKDI
61ILRTEQNSGR TILIKAVTEK NFETKDFRAS LENGVLLCDL INKLKPGVIK KINRLSTPIA
121GLDNINVFLK ACEQIGLKEA QLFHPGDLQD LSNRVTVKQE ETDRRVKNVL ITLYWLGRKA
181QSNPYYNGPH LNLKAFENLL GQALTKALED SSFLKRSGRD SGYGDIWCPE RGEFLAPPRH
241HKREDSFESL DSLGSRSLTS CSSDITLRGG REGFESDTDS EFTFKMQDYN KDDMSYRRIS
301AVEPKTALPF NRFLPNKSRQ PSYVPAPLRK KKPDKHEDNR RSWASPVYTE ADGTFSRLFQ
361KIYGENGSKS MSDVSAEDVQ NLRQLRYEEM QKIKSQLKEQ DQKWQDDLAK WKDRRKSYTS
421DLQKKKEERE EIEKQALEKS KRSSKTFKEM LQDRESQNQK STVPSRRRMY SFDDVLEEGK
481RPPTMTVSEA SYQSERVEEK GATYPSEIPK EDSTTFAKRE DRVTTEIQLP SQSPVEEQSP
541ASLSSLRSRS TQMESTCVSA SLPRSYRKTD TVRLTSVVTP RPFGSQTRGI SSLPRSYTMD
601DAWKYNGDIE DIKRTPNNVV STPAPSPDAS QLASSLSSQK EVAATEEDVT RLPSPTSPFS
661SLSQDQAATS KATLSSTSGL DLMSESGEGE ISPQREVSRS QDQFSDMRIS INQTPGKSLD
721FGFTIKWDIP GIFVASVEAG SPAEFSQLQV DDEIIAINNT KFSYNDSKEW EEAMAKAQET
781GHLVMDVRRY GKAGSPETKW IDATSGIYNS EKSSNLSVTT DFSESLQSSN IESKEINGIH
841DESNAFESKA SESISLKNLK RRSQFFEQGS SDSVVPDLPV PTISAPSRWV WDQEEERKRQ
901ERWQKEQDRL LQEKYQREQE KLREEWQRAK QEAERENSKY LDEELMVLSS NSMSLTTREP
961SLATWEATWS EGSKSSDREG TRAGEEERRQ PQEEVVHEDQ GKKPQDQLVI ERERKWEQQL
1021QEEQEQKRLQ AEAEEQKRPA EEQKRQAEIE RETSVRIYQY RRPVDSYDIP KTEEASSGFL
1081PGDRNKSRST TELDDYSTNK NGNNKYLDQI GNTTSSQRRS KKEQVPSGAE LERQQILQEM
1141RKRTPLHNDN SWIRQRSASV NKEPVSLPGI MRRGESLDNL DSPRSNSWRQ PPWLNQPTGF
1201YASSSVQDFS RPPPQLVSTS NRAYMRNPSS SVPPPSAGSV KTSTTGVATT QSPTPRSHSP
1261SASQSGSQLR NRSVSGKRIC SYCNNILGKG AAMIIESLGL CYHLHCFKCV ACECDLGGSS
1321SGAEVRIRNH QLYCNDCYLR FKSGRPTAM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1349 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.