Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LMNTD2 / C11orf35 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105222-100
Catalog Number: LS-G105222
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LMNTD2 (NM_173573), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_173573
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 70.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRWLRPAGRR REQESVSGHL GPPAGAPAAP ETPTCLPDTT PHPTPVVCSA DPQLALESLD61PRTLRLLWRQ RELEIQALRW AIQNGEDARL CHILEEVAGL PPKRSSHSQE KLLQNQVQKL121IQELKEQKER AQWEKEHLEE RLLQTTRTLQ EMEAELQNLQ KSCLLQLARS SWVGRMLRSQ181TGSVEVVTAE TLMDPSDLSE NIQAPTGEGF RLEDVDWNSV ARRYPNLFTN MEPSSKQKQP241RPWPQLDTGS PESSGKHSER HHKTVEWGSL PCLNTSSSGG ADSDSSSCRP GLPSFVQVIG301HPPRDHRASS EQALVQAGSY SRDSEDLQKT HSPRHGEPVL SPQPCTDPDH WSPELLQSPT361GLKIVAVSCR EKFVRIFNPS QESTADLSGM VLKQLVRGFP ERLYRFPPGT LLAPRHHVTV421WGEATRSAKK PLRASSSREP VPLLSIRGCA TLLLSPKGEV LSEHRIPRRE TPAPRVFADG481TDLSIDRFPL PEAGPGADTR KPPRPPRPLR KGRVREPRVS RRRPGTRGLL PPVSSGKLFH541AREGPARPEN PEIPAPQHLP AIPGDPTLPS PPAEAGLGLE DCRLQKEHRV RVCRKSVDRS601CPLVALSVQN TAESRFGFRF LSCLPVTADT CRGATRTRPLEQKLISEEDLAANDILDYKDDDDKV
634 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.