Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LMNTD1 / IFLTD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126815-100
Catalog Number: LS-G126815
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAS1C1(LMNTD1) (NM_001145727), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IFLTD1 | PAS1C1
- Origin Species
- Human
- SwissProt
- Q8N9Z9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MQQPVRAGDI GVSRVQTHHN ASPFSVPKKQ DESPMIGDGE DYFLSLFGDS KKLTAHSNYT61QKTLKYFSMI LEEVGQFTSS SLGDVEIAEV NVKGLFVKLI NSSLDKEMAI GDHILQQNVN121GQTISLYRFL PNIVMQANST VTVWAAASEA KHQPPSDFLW KEQDKFRASP DCITILCKPN181GQAIAWYTPI HWKQAWEKLD ADVEFNRCSV VSPTFRKRVF QWTASTATIT KEKQDQPKKD241ISNYQVEQAQ VLLKREKEIP PTVFPNRSPW CQNPYVSAHP YCPLIEPHNT STAGGRLDRQ301PRTRSTRPNR ASGSKKKKTS ESQKQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
325 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.