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LMLN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LMLN Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LMLN antibodies →
$725.00each
Catalog Number: LS-G125634-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Invadolysin(LMLN) (NM_001136049), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001136049

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVTTLGPKMA AEWGGGVGYS GSGPGRSRWR WSGSVWVRSV LLLLGGLRAS ATSTPVSLGS
61SPPCRHHVPS DTEVINKVHL KANHVVKRDV DEHLRIKTVY DKSVEELLPE KKNLVKNKLF
121PQAISYLEKT FQVRRPAGTI LLSRQCATNQ YLRKENDPHR YCTGECAAHT KCGPVIVPEE
181HLQQCRVYRG GKWPHGAVGV PDQEGISDAD FVLYVGALAT ERCSHENIIS YAAYCQQEAN
241MDRPIAGYAN LCPNMISTQP QEFVGMLSTV KHEVIHALGF SAGLFAFYHD KDGNPLTSRF
301ADGLPPFNYS LGLYQWSDKV VRKVERLWDV RDNKIVRHTV YLLVTPRVVE EARKHFDCPV
361LEGMELENQG GVGTELNHWE KRLLENEAMT GSHTQNRVLS RITLALMEDT GWYKANYSMA
421EKLDWGRGMG CDFVRKSCKF WIDQQRQKRQ MLSPYCDTLR SNPLQLTCRQ DQRAVAVCNL
481QKFPKPLPQE YQYFDELSGI PAEDLPYYGG SVEIADYCPF SQEFSWHLSG EYQRSSDCRI
541LENQPEIFKN YGAEKYGPHS VCLIQKSAFV MEKCERKLSY PDWGSGCYQV SCSPQGLKVW
601VQDTSYLCSR AGQVLPVSIQ MNGWIHDGNL LCPSCWDFCE LCPPETDPPA TNLTRALPLD
661LCSCSSSLVV TLWLLLGNLF PLLAGFLLCI WH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

692 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.