Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LMAN2 / VIP36 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115067-100
Catalog Number: LS-G115067
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LMAN2 (NM_006816), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C5orf8 | Glycoprotein GP36b | gp36B | Lectin mannose-binding 2 | Lectin | mannose-binding 2 | LMAN2 | VIP36
- Origin Species
- Human
- NCBI GenBank
- NM_006816
- SwissProt
- Q12907
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.23 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAEGWIWRW GWGRRCLGRP GLLGPGPGPT TPLFLLLLLG SVTADITDGN SEHLKREHSL61IKPYQGVGSS SMPLWDFQGS TMLTSQYVRL TPDERSKEGS IWNHQPCFLK DWEMHVHFKV121HGTGKKNLHG DGIALWYTRD RLVPGPVFGS KDNFHGLAIF LDTYPNDETT ERVFPYISVM181VNNGSLSYDH SKDGRWTELA GCTADFRNRD HDTFLAVRYS RGRLTVMTDL EDKNEWKNCI241DITGVRLPTG YYFGASAGTG DLSDNHDIIS MKLFQLMVEH TPDEESIDWT KIEPSVNFLK301SPKDNVDDPT GNFRSGPLTG WRVFLLLLCA LLGIVVCAVV GAVVFQKRQE RNKRFYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
356 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.