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LMAN1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LMAN1L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110589-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LMAN1L (NM_021819), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ERGIC-53-like protein | ERGIC53-like protein | ERGIC-53L | LMAN1-like protein | LMAN1L | Protein ERGIC-53-like | ERGL | Lectin mannose-binding 1-like
Origin Species
Human
NCBI GenBank
NM_021819
SwissProt
Q9HAT1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPAVSGPGPL FCLLLLLLDP HSPETGCPPL RRFEYKLSFK GPRLALPGAG IPFWSHHGDA
61ILGLEEVRLT PSMRNRSGAV WSRASVPFSA WEVEVQMRVT GLGRRGAQGM AVWYTRGRGH
121VGSVLGGLAS WDGIGIFFDS PAEDTQDSPA IRVLASDGHI PSEQPGDGAS QGLGSCHWDF
181RNRPHPFRAR ITYWGQRLRM SLNSGLTPSD PGEFCVDVGP LLLVPGGFFG VSAATGTLAD
241DHDVLSFLTF SLSEPSPEVP PQPFLEMQQL RLARQLEGLW ARLGLGTRED VTPKSDSEAQ
301GEGERLFDLE ETLGRHRRIL QALRGLSKQL AQAERQWKKQ LGPPGQARPD GGWALDASCQ
361IPSTPGRGGH LSMSLNKDSA KVGALLHGQW TLLQALQEMR DAAVRMAAEA QVSYLPVGIE
421HHFLELDHIL GLLQEELRGP AKAAAKAPRP PGQPPRASSC LQPGIFLFYL LIQTVGFFGY
481VHFRQELNKS LQECLSTGSL PLGPAPHTPR ALGILRRQPL PASMPA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

526 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.