Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LIPM / LIPL3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G124778-100
Catalog Number: LS-G124778
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LIPM (NM_001128215), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA304I5.1 | Lipase M | LIPM | Lipase member M | Lipase | family member M | LIPL3
- Origin Species
- Human
- SwissProt
- Q5VYY2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLETLSRQWI VSHRMEMWLL ILVAYMFQRN VNSVHMPTKA VDPEAFMNIS EIIQHQGYPC61EEYEVATEDG YILSVNRIPR GLVQPKKTGS RPVVLLQHGL VGGASNWISN LPNNSLGFIL121ADAGFDVWMG NSRGNAWSRK HKTLSIDQDE FWAFSYDEMA RFDLPAVINF ILQKTGQEKI181YYVGYSQGTT MGFIAFSTMP ELAQKIKMYF ALAPIATVKH AKSPGTKFLL LPDMMIKGLF241GKKEFLYQTR FLRQLVIYLC GQVILDQICS NIMLLLGGFN TNNMNMSRAS VYAAHTLAGT301SVQNILHWSQ AVNSGELRAF DWGSETKNLE KCNQPTPVRY RVRDMTVPTA MWTGGQDWLS361NPEDVKMLLS EVTNLIYHKN IPEWAHVDFI WGLDAPHRMY NEIIHLMQQE ETNLSQGRCE421AVLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
423 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.