Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LIPF / GL / Gastric Lipase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100232-100
Catalog Number: LS-G100232
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LIPF (NM_004190), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Gastric lipase | Gastric triacylglycerol lipase | GL | LIPF | HLAL | HGL | Lipase | gastric
- Origin Species
- Human
- NCBI GenBank
- NM_004190
- SwissProt
- P07098
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.24 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWLLLTMASL ISVLGTTHGL FGKLHPGSPE VTMNISQMIT YWGYPNEEYE VVTEDGYILE61VNRIPYGKKN SGNTGQRPVV FLQHGLLASA TNWISNLPNN SLAFILADAG YDVWLGNSRG121NTWARRNLYY SPDSVEFWAF SFDEMAKYDL PATIDFIVKK TGQKQLHYVG HSQGTTIGFI181AFSTNPSLAK RIKTFYALAP VATVKYTKSL INKLRFVPQS LFKFIFGDKI FYPHNFFDQF241LATEVCSREM LNLLCSNALF IICGFDSKNF NTSRLDVYLS HNPAGTSVQN MFHWTQAVKS301GKFQAYDWGS PVQNRMHYDQ SQPPYYNVTA MNVPIAVWNG GKDLLADPQD VGLLLPKLPN361LIYHKEIPFY NHLDFIWAMD APQEVYNDIV SMISEDKKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
398 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.