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LINGO3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LINGO3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G124053-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LINGO3 (NM_001101391), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LERN2 | LRRN6B | LINGO3 | HCG2040376
Origin Species
Human
NCBI GenBank
NM_001101391
SwissProt
P0C6S8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTCWLCVLSL PLLLLPAAPP PAGGCPARCE CTVQTRAVAC TRRRLTAVPD GIPAETRLLE
61LSRNRIRCLN PGDLAALPAL EELDLSENAI AHVEPGAFAN LPRLRVLRLR GNQLKLIPPG
121VFTRLDNLTL LDLSENKLVI LLDYTFQDLH SLRRLEVGDN DLVFVSRRAF AGLLALEELT
181LERCNLTALS GESLGHLRSL GALRLRHLAI ASLEDQNFRR LPGLLHLEID NWPLLEEVAA
241GSLRGLNLTS LSVTHTNITA VPAAALRHQA HLTCLNLSHN PISTVPRGSF RDLVRLRELH
301LAGALLAVVE PQAFLGLRQI RLLNLSNNLL STLEESTFHS VNTLETLRVD GNPLACDCRL
361LWIVQRRKTL NFDGRLPACA TPAEVRGDAL RNLPDSVLFE YFVCRKPKIR ERRLQRVTAT
421AGEDVRFLCR AEGEPAPTVA WVTPQHRPVT ATSAGRARVL PGGTLEIQDA RPQDSGTYTC
481VASNAGGNDT YFATLTVRPE PAANRTPGEA HNETLAALRA PLDLTTILVS TAMGCITFLG
541VVLFCFVLLF VWSRGRGQHK NNFSVEYSFR KVDGPAAAAG QGGARKFNMK MI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

592 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.