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LINGO2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LINGO2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106165-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LINGO2 (NM_152570), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LERN3 | LINGO2 | LRRN6C
Origin Species
Human
NCBI GenBank
NM_152570
SwissProt
Q7L985

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLHTAISCWQ PFLGLAVVLI FMGSTIGCPA RCECSAQNKS VSCHRRRLIA IPEGIPIETK
61ILDLSKNRLK SVNPEEFISY PLLEEIDLSD NIIANVEPGA FNNLFNLRSL RLKGNRLKLV
121PLGVFTGLSN LTKLDISENK IVILLDYMFQ DLHNLKSLEV GDNDLVYISH RAFSGLLSLE
181QLTLEKCNLT AVPTEALSHL RSLISLHLKH LNINNMPVYA FKRLFHLKHL EIDYWPLLDM
241MPANSLYGLN LTSLSVTNTN LSTVPFLAFK HLVYLTHLNL SYNPISTIEA GMFSDLIRLQ
301ELHIVGAQLR TIEPHSFQGL RFLRVLNVSQ NLLETLEENV FSSPRALEVL SINNNPLACD
361CRLLWILQRQ PTLQFGGQQP MCAGPDTIRE RSFKDFHSTA LSFYFTCKKP KIREKKLQHL
421LVDEGQTVQL ECSADGDPQP VISWVTPRRR FITTKSNGRA TVLGDGTLEI RFAQDQDSGM
481YVCIASNAAG NDTFTASLTV KGFASDRFLY ANRTPMYMTD SNDTISNGTN ANTFSLDLKT
541ILVSTAMGCF TFLGVVLFCF LLLFVWSRGK GKHKNSIDLE YVPRKNNGAV VEGEVAGPRR
601FNMKMI
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

606 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.