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LINGO1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LINGO1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G108630-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LINGO1 (NM_032808), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LERN1 | LINGO1 | LRRN6A | UNQ201
Origin Species
Human
NCBI GenBank
NM_032808
SwissProt
Q96FE5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQVSKRMLAG GVRSMPSPLL ACWQPILLLV LGSVLSGSAT GCPPRCECSA QDRAVLCHRK
61RFVAVPEGIP TETRLLDLGK NRIKTLNQDE FASFPHLEEL ELNENIVSAV EPGAFNNLFN
121LRTLGLRSNR LKLIPLGVFT GLSNLTKLDI SENKIVILLD YMFQDLYNLK SLEVGDNDLV
181YISHRAFSGL NSLEQLTLEK CNLTSIPTEA LSHLHGLIVL RLRHLNINAI RDYSFKRLYR
241LKVLEISHWP YLDTMTPNCL YGLNLTSLSI THCNLTAVPY LAVRHLVYLR FLNLSYNPIS
301TIEGSMLHEL LRLQEIQLVG GQLAVVEPYA FRGLNYLRVL NVSGNQLTTL EESVFHSVGN
361LETLILDSNP LACDCRLLWV FRRRWRLNFN RQQPTCATPE FVQGKEFKDF PDVLLPNYFT
421CRRARIRDRK AQQVFVDEGH TVQFVCRADG DPPPAILWLS PRKHLVSAKS NGRLTVFPDG
481TLEVRYAQVQ DNGTYLCIAA NAGGNDSMPA HLHVRSYSPD WPHQPNKTFA FISNQPGEGE
541ANSTRATVPF PFDIKTLIIA TTMGFISFLG VVLFCLVLLF LWSRGKGNTK HNIEIEYVPR
601KSDAGISSAD APRKFNMKMI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

620 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.