LSBio
LIN9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LIN9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LIN9 antibodies →
$504.00each
Catalog Number: LS-G105410-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LIN9 (NM_173083), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BARPsv | Lin-9 | LIN9 | Lin-9 homolog (C. elegans) | HLin-9 | PRB-associated protein | Rb related pathway actor | TGS | TUDOR gene similar protein | Protein lin-9 homolog | BARA | HuLin-9 | TGS2
Origin Species
Human
NCBI GenBank
AA256253
SwissProt
Q5TKA1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MHRGGQPLKK RRGSFKMAEL DQLPDESSSA KALVSLKEGS LSNTWNEKYS SLQKTPVWKG
61RNTSSAVEMP FRNSKRSRLF SDEDDRQINT RSPKRNQRVA MVPQKFTATM STPDKKASQK
121IGFRLRNLLK LPKAHKWCIY EWFYSNIDKP LFEGDNDFCV CLKESFPNLK TRKLTRVEWG
181KIRRLMGKPR RCSSAFFEEE RSALKQKRQK IRLLQQRKVA DVSQFKDLPD EIPLPLVIGT
241KVTARLRGVH DGLFTGQIDA VDTLNATYRV TFDRTGLGTH TIPDYEVLSN EPHETMPIAA
301FGQKQRPSRF FMTPPRLHYT PPLQSPIIDN DPLLGQSPWR SKISGSDTET LGGFPVEFLI
361QVTRLSKILM IKKEHIKKLR EMNTEAEKLK SYSMPISIEF QRRYATIVLE LEQLNKDLNK
421VLHKVQQYCY ELAPDQGLQP ADQPTDMRRR CEEEAQEIVR HANSSTGQPC VENENLTDLI
481SRLTAILLQI KCLAEGGDLN SFEFKSLTDS LNDIKSTIDA SNISCFQNNV EIHVAHIQSG
541LSQMGNLHAF AANNTNRD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

558 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.