Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LIN41 / TRIM71 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120568-100
Catalog Number: LS-G120568
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRIM71 (NM_001039111), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Abnormal cell LINeage LIN-41 | Homolog of C. elegans Lin-41 | Protein lin-41 homolog | LIN41 | TRIM71 | Tripartite motif containing 71 | Tripartite motif-containing 71 | LIN-41
- Origin Species
- Human
- SwissProt
- Q2Q1W2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 93.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASFPETDFQ ICLLCKEMCG SPAPLSSNSS ASSSSSQTST SSGGGGGGPG AAARRLHVLP61CLHAFCRPCL EAHRLPAAGG GAAGEPLKLR CPVCDQKVVL AEAAGMDALP SSAFLLSNLL121DAVVATADEP PPKNGRAGAP AGAGGHSNHR HHAHHAHPRA SASAPPLPQA PQPPAPSRSA181PGGPAASPSA LLLRRPHGCS SCDEGNAASS RCLDCQEHLC DNCVRAHQRV RLTKDHYIER241GPPGPGAAAA AQQLGLGPPF PGPPFSILSV FPERLGFCQH HDDEVLHLYC DTCSVPICRE301CTMGRHGGHS FIYLQEALQD SRALTIQLLA DAQQGRQAIQ LSIEQAQTVA EQVEMKAKVV361QSEVKAVTAR HKKALEEREC ELLWKVEKIR QVKAKSLYLQ VEKLRQNLNK LESTISAVQQ421VLEEGRALDI LLARDRMLAQ VQELKTVRSL LQPQEDDRVM FTPPDQALYL AIKSFGFVSS481GAFAPLTKAT GDGLKRALQG KVASFTVIGY DHDGEPRLSG GDLMSAVVLG PDGNLFGAEV541SDQQNGTYVV SYRPQLEGEH LVSVTLCNQH IENSPFKVVV KSGRSYVGIG LPGLSFGSEG601DSDGKLCRPW GVSVDKEGYI IVADRSNNRI QVFKPCGAFH HKFGTLGSRP GQFDRPAGVA661CDASRRIVVA DKDNHRIQIF TFEGQFLLKF GEKGTKNGQF NYPWDVAVNS EGKILVSDTR721NHRIQLFGPD GVFLNKYGFE GALWKHFDSP RGVAFNHEGH LVVTDFNNHR LLVIHPDCQS781ARFLGSEGTG NGQFLRPQGV AVDQEGRIIV ADSRNHRVQM FESNGSFLCK FGAQGSGFGQ841MDRPSGIAIT PDGMIVVVDF GNNRILVFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
868 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.