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LIMK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LIMK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112502-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LIM kinase 2(LIMK2) (NM_016733), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LIM domain kinase 2 | LIMK2 | LIM kinase 2 | LIMK-2
Origin Species
Human
NCBI GenBank
NM_016733
SwissProt
P53671

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSYLSVPAY FTSRDLFRCS ECQDSLTNWY YEKDGKLYCP KDYWGKFGEF CHGCSLLMTG
61PFMVAGEFKY HPECFACMSC KVIIEDGDAY ALVQHATLYC GKCHNEVVLA PMFERLSTES
121VQEQLPYSVT LISMPATTEG RRGFSVSVES ACSNYATTVQ VKEVNRMHIS PNNRNAIHPG
181DRILEINGTP VRTLRVEEVE DAISQTSQTL QLLIEHDPVS QRLDQLRLEA RLAPHMQNAG
241HPHALSTLDT KENLEGTLRR RSLRRSNSIS KSPGPSSPKE PLLFSRDISR SESLRCSSSY
301SQQIFRPCDL IHGEVLGKGF FGQAIKVTHK ATGKVMVMKE LIRCDEETQK TFLTEVKVMR
361SLDHPNVLKF IGVLYKDKKL NLLTEYIEGG TLKDFLRSMD PFPWQQKVRF AKGIASGMAY
421LHSMCIIHRD LNSHNCLIKL DKTVVVADFG LSRLIVEERK RAPMEKATTK KRTLRKNDRK
481KRYTVVGNPY WMAPEMLNGK SYDETVDIFS FGIVLCEIIG QVYADPDCLP RTLDFGLNVK
541LFWEKFVPTD CPPAFFPLAA ICCRLEPESR PAFSKLEDSF EALSLYLGEL GIPLPAELEE
601LDHTVSMQYG LTRDSPP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

617 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.