Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LILRB2 / ILT4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119734-100
Catalog Number: LS-G119734
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LILRB2 (NM_001080978), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD85D | CD85d antigen | Ig-like transcript 4 | LIR-2 | MIR-10 | MIR10 | LILRB2 | ILT-4 | ILT4 | LIR2
- Origin Species
- Human
- NCBI GenBank
- NM_005874
- SwissProt
- Q8N423
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTPIVTVLIC LGLSLGPRTH VQTGTIPKPT LWAEPDSVIT QGSPVTLSCQ GSLEAQEYRL61YREKKSASWI TRIRPELVKN GQFHIPSITW EHTGRYGCQY YSRARWSELS DPLVLVMTGA121YPKPTLSAQP SPVVTSGGRV TLQCESQVAF GGFILCKEGE DEHPQCLNSQ PHARGSSRAI181FSVGPVSPNR RWSHRCYGYD LNSPYVWSSP SDLLELLVPG VSKKPSLSVQ PGPVVAPGES241LTLQCVSDVG YDRFVLYKEG ERDLRQLPGR QPQAGLSQAN FTLGPVSRSY GGQYRCYGAY301NLSSEWSAPS DPLDILITGQ IHGTPFISVQ PGPTVASGEN VTLLCQSWRQ FHTFLLTKAG361AADAPLRLRS IHEYPKYQAE FPMSPVTSAH AGTYRCYGSL NSDPYLLSHP SEPLELVVSG421PSMGSSPPPT GPISTPAGPE DQPLTPTGSD PQSGLGRHLG VVIGILVAVV LLLLLLLLLF481LILRHRRQGK HWTSTQRKAD FQHPAGAVGP EPTDRGLQWR SSPAADAQEE NLYAAVKDTQ541PEDGVEMDTR AAASEAPQDV TYAQLHSLTL RRKATEPPPS QEGEPPAEPS IYATLAIHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
598 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.