Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LILRA1 / LIR6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115034-100
Catalog Number: LS-G115034
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LILRA1 (NM_006863), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD85I | CD85i antigen | LILRA1 | LIR-6 | LIR6
- Origin Species
- Human
- NCBI GenBank
- NM_006863
- SwissProt
- O75019
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTPIVTVLIC LRLSLGPRTH VQAGTLPKPT LWAEPGSVIT QGSPVTLWCQ GILETQEYRL61YREKKTAPWI TRIPQEIVKK GQFPIPSITW EHTGRYRCFY GSHTAGWSEP SDPLELVVTG121AYIKPTLSAL PSPVVTSGGN VTLHCVSQVA FGSFILCKEG EDEHPQCLNS QPRTHGWSRA181IFSVGPVSPS RRWSYRCYAY DSNSPHVWSL PSDLLELLVL GVSKKPSLSV QPGPIVAPGE241SLTLQCVSDV SYDRFVLYKE GERDFLQLPG PQPQAGLSQA NFTLGPVSRS YGGQYRCSGA301YNLSSEWSAP SDPLDILIAG QFRGRPFISV HPGPTVASGE NVTLLCQSWG PFHTFLLTKA361GAADAPLRLR SIHEYPKYQA EFPMSPVTSA HSGTYRCYGS LSSNPYLLSH PSDSLELMVS421GAAETLSPPQ NKSDSKAGAA NTLSPSQNKT ASHPQDYTVE NLIRMGIAGL VLVVLGILLF481EAQHSQRSLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
489 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.