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LGR6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LGR6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LGR6 antibodies →
$725.00each
Catalog Number: LS-G127166-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LGR6 (NM_021636), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
VTS20631 | LGR6
Origin Species
Human
NCBI GenBank
AB049405
SwissProt
Q9HBX8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGRPRLTLVC QVSIIISARD LSMNNLTELQ PGLFHHLRFL EELRLSGNHL SHIPGQAFSG
61LYSLKILMLQ NNQLGGIPAE ALWELPSLQS LRLDANLISL VPERSFEGLS SLRHLWLDDN
121ALTEIPVRAL NNLPALQAMT LALNRISHIP DYAFQNLTSL VVLHLHNNRI QHLGTHSFEG
181LHNLETLDLN YNKLQEFPVA IRTLGRLQEL GFHNNNIKAI PEKAFMGNPL LQTIHFYDNP
241IQFVGRSAFQ YLPKLHTLSL NGAMDIQEFP DLKGTTSLEI LTLTRAGIRL LPSGMCQQLP
301RLRVLELSHN QIEELPSLHR CQKLEEIGLQ HNRIWEIGAD TFSQLSSLQA LDLSWNAIRS
361IHPEAFSTLH SLVKLDLTDN QLTTLPLAGL GGLMHLKLKG NLALSQAFSK DSFPKLRILE
421VPYAYQCCPY GMCASFFKAS GQWEAEDLHL DDEESSKRPL GLLARQAENH YDQDLDELQL
481EMEDSKPHPS VQCSPTPGPF KPCEYLFESW GIRLAVWAIV LLSVLCNGLV LLTVFAGGPV
541PLPPVKFVVG AIAGANTLTG ISCGLLASVD ALTFGQFSEY GARWETGLGC RATGFLAVLG
601SEASVLLLTL AAVQCSVSVS CVRAYGKSPS LGSVRAGVLG CLALAGLAAA LPLASVGEYG
661ASPLCLPYAP PEGQPAALGF TVALVMMNSF CFLVVAGAYI KLYCDLPRGD FEAVWDCAMV
721RHVAWLIFAD GLLYCPVAFL SFASMLGLFP VTPEAVKSVL LVVLPLPACL NPLLYLLFNP
781HFRDDLRRLR PRAGDSGPLA YAAAGELEKS SCDSTQALVA FSDVDLILEA SEAGRPPGLE
841TYGFPSVTLI SCQQPGAPRL EGSHCVEPEG NHFGNPQPSM DGELLLRAEG STPAGGGLSG
901GGGFQPSGLA FASHV
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

915 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.