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LGI4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LGI4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LGI4 antibodies →
$725.00each
Catalog Number: LS-G107050-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LGI4 (NM_139284), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LGI1-like protein 3 | LGIL3 | LGI4
Origin Species
Human
SwissProt
Q8N135

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGGAGILLLL LAGAGVVVAW RPPKGKCPLR CSCSKDSALC EGSPDLPVSF SPTLLSLSLV
61RTGVTQLKAG SFLRIPSLHL LLFTSNSFSV IEDDAFAGLS HLQYLFIEDN EIGSISKNAL
121RGLRSLTHLS LANNHLETLP RFLFRGLDTL THVDLRGNPF QCDCRVLWLL QWMPTVNASV
181GTGACAGPAS LSHMQLHHLD PKTFKCRAIE LSWFQTVGES ALSVEPFSYQ GEPHIVLAQP
241FAGRCLILSW DYSLQRFRPE EELPAASVVS CKPLVLGPSL FVLAARLWGG SQLWARPSPG
301LRLAPTQTLA PRRLLRPNDA ELLWLEGQPC FVVADASKAG STTLLCRDGP GFYPHQSLHA
361WHRDTDAEAL ELDGRPHLLL ASASQRPVLF HWTGGRFERR TDIPEAEDVY ATRHFQAGGD
421VFLCLTRYIG DSMVMRWDGS MFRLLQQLPS RGAHVFQPLL IARDQLAILG SDFAFSQVLR
481LEPDKGLLEP LQELGPPALV APRAFAHITM AGRRFLFAAC FKGPTQIYQH HEIDLSA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

537 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.