LSBio
LGI3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LGI3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LGI3 antibodies →
$725.00each
Catalog Number: LS-G107047-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LGI3 (NM_139278), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LGIL4 | LGI1-like protein 4 | LGI3
Origin Species
Human
NCBI GenBank
NM_139278
SwissProt
Q8N145

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGLRARGGP GPGLLALSAL GFCLMLQVSA KRPPKTPPCP PSCSCTRDTA FCVDSKAVPR
61NLPSEVISLT LVNAAFSEIQ DGAFSHLPLL QFLLLNSNKF TLIGDNAFTG LSHLQYLFIE
121NNDIWALSKF TFRGLKSLTH LSLANNNLQT LPRDIFRPLD ILNDLDLRGN SLNCDCKVKW
181LVEWLAHTNT TVAPIYCASP PRFQEHKVQD LPLREFDCIT TDFVLYQTLA FPAVSAEPFL
241YSSDLYLALA QPGVSACTIL KWDYVERQLR DYDRIPAPSA VHCKPMVVDS QLYVVVAQLF
301GGSYIYHWDP NTTRFTRLQD IDPQRVRKPN DLEAFRIDGD WYFAVADSSK AGATSLYRWH
361QNGFYSHQAL HPWHRDTDLE FVDGEGKPRL IVSSSSQAPV IYQWSRTQKQ FVAQGEVTQV
421PDAQAVKHFR AGRDSYLCLS RYIGDSKILR WEGTRFSEVQ ALPSRGSLAL QPFLVGGRRY
481LALGSDFSFT QIYQWDEGRQ KFVRFQELAV QAPRAFCYMP AGDAQLLLAP SFKGQTLVYR
541HIVVDLSA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

548 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.