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LGI2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LGI2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101553-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LGI2 (NM_018176), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1916 | LGI1-like protein 2 | LGI2 | LGIL2
Origin Species
Human
NCBI GenBank
NM_018176
SwissProt
Q8N0V4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALRRGGCGA LGLLLLLLGA ACLIPRSAQV RRLARCPATC SCTKESIICV GSSWVPRIVP
61GDISSLSLVN GTFSEIKDRM FSHLPSLQLL LLNSNSFTII RDDAFAGLFH LEYLFIEGNK
121IETISRNAFR GLRDLTHLSL ANNHIKALPR DVFSDLDSLI ELDLRGNKFE CDCKAKWLYL
181WLKMTNSTVS DVLCIGPPEY QEKKLNDVTS FDYECTTTDF VVHQTLPYQS VSVDTFNSKN
241DVYVAIAQPS MENCMVLEWD HIEMNFRSYD NITGQSIVGC KAILIDDQVF VVVAQLFGGS
301HIYKYDESWT KFVKFQDIEV SRISKPNDIE LFQIDDETFF VIADSSKAGL STVYKWNSKG
361FYSYQSLHEW FRDTDAEFVD IDGKSHLILS SRSQVPIILQ WNKSSKKFVP HGDIPNMEDV
421LAVKSFRMQN TLYLSLTRFI GDSRVMRWNS KQFVEIQALP SRGAMTLQPF SFKDNHYLAL
481GSDYTFSQIY QWDKEKQLFK KFKEIYVQAP RSFTAVSTDR RDFFFASSFK GKTKIFEHII
541VDLSL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

545 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.