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Leukotriene B4 Receptor / BLT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Leukotriene B4 Receptor / BLT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LTB4R antibodies →
$504.00each
Catalog Number: LS-G126241-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Leukotriene B4 Receptor(LTB4R) (NM_001143919), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BLT | BLT1 | BLTR | Blt-1 | Chemokine receptor-like 1 | G protein-coupled receptor 16 | GPR16 | Leukotriene B4 receptor | Leukotriene B4 receptor 1 | G-protein coupled receptor 16 | LTBR1 | LTB4R | p2Y purinoceptor 7 | p2Y7 | LTB4-R 1 | p2RY7 | CMKRL1 | LTB4-R1 | LTB4R1
Origin Species
Human
NCBI GenBank
D89079
SwissProt
Q15722

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
37.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MNTTSSAAPP SLGVEFISLL AIILLSVALA VGLPGNSFVV WSILKRMQKR SVTALMVLNL
61ALADLAVLLT APFFLHFLAQ GTWSFGLAGC RLCHYVCGVS MYASVLLITA MSLDRSLAVA
121RPFVSQKLRT KAMARRVLAG IWVLSFLLAT PVLAYRTVVP WKTNMSLCFP RYPSEGHRAF
181HLIFEAVTGF LLPFLAVVAS YSDIGRRLQA RRFRRSRRTG RLVVLIILTF AAFWLPYHVV
241NLAEAGRALA GQAAGLGLVG KRLSLARNVL IALAFLSSSV NPVLYACAGG GLLRSAGVGF
301VAKLLEGTGS EASSTRRGGS LGQTARSGPA ALEPGPSESL TASSPFKLNE LN
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

352 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.