Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LEREPO4 / ZC3H15 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111725-100
Catalog Number: LS-G111725
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZC3H15 (NM_018471), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DFRP1 | HT010 | MSTP012 | ZC3H15 | LEREPO4
- Origin Species
- Human
- NCBI GenBank
- NM_018471
- SwissProt
- Q8WU90
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPPKKQAQAG GSKKAEQKKK EKIIEDKTFG LKNKKGAKQQ KFIKAVTHQV KFGQQNPRQV61AQSEAEKKLK KDDKKKELQE LNELFKPVVA AQKISKGADP KSVVCAFFKQ GQCTKGDKCK121FSHDLTLERK CEKRSVYIDA RDEELEKDTM DNWDEKKLEE VVNKKHGEAE KKKPKTQIVC181KHFLEAIENN KYGWFWVCPG GGDICMYRHA LPPGFVLKKD KKKEEKEDEI SLEDLIERER241SALGPNVTKI TLESFLAWKK RKRQEKIDKL EQDMERRKAD FKAGKALVIS GREVFEFRPE301LVNDDDEEAD DTRYTQGTGG DEVDDSVSVN DIDLSLYIPR DVDETGITVA SLERFSTYTS361DKDENKLSEA SGGRAENGER SDLEEDNERE GTENGAIDAV PVDENLFTGE DLDELEEELN421TLDLEETRTRPLEQKLISEEDLAANDILDYKDDDDKV
426 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.