LSBio
LENG8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LENG8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LENG8 antibodies →
$504.00each
Catalog Number: LS-G108085-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LENG8 (NM_052925), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LENG8 | KIAA1932 | Pp13842
Origin Species
Human
NCBI GenBank
AA075783

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
88 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAANVGDQRS TDWSSQYSMV AGAGRENGME TPMHENPEWE KARQALASIS KSGAAGGSAK
61SSSNGPVASA QYVSQAEASA LQQQQYYQWY QQYNYAYPYS YYYPMSMYQS YGSPSQYGMA
121GSYGSATPQQ PSAPQHQGTL NQPPVPGMDE SMSYQAPPQQ LPSAQPPQPS NPPHGAHTLN
181SGPQPGTAPA TQHSQAGPAT GQAYGPHTYT EPAKPKKGQQ LWNRMKPAPG TGGLKFNIQK
241RPFAVTTQSF GSNAEGQHSG FGPQPNPEKV QNHSGSSARG NLSGKPDDWP QDMKEYVERC
301FTACESEEDK DRTEKLLKEV LQARLQDGSA YTIDWSREPL PGLTREPVAE SPKKKRWEAA
361SSLHPPRGAG SATRGGGAPS QRGTPGAGGA GRARGNSFTK FGNRNVFMKD NSSSSSTDSR
421SRSSSRSPTR HFRRSDSHSD SDSSYSGNEC HPVGRRNPPP KGRGGRGAHM DRGRGRAQRG
481KRHDLAPTKR SRKKMAALEC EDPERELKKQ KRAARFQHGH SRRLRLEPLV LQMSSLESSG
541ADPDWQELQI VGTCPDITKH YLRLTCAPDP STVRPVAVLK KSLCMVKCHW KEKQDYAFAC
601EQMKSIRQDL TVQGIRTEFT VEVYETHARI ALEKGDHEEF NQCQTQLKSL YAENLPGNVG
661EFTAYRILYY IFTKNSGDIT TELAYLTREL KADPCVAHAL ALRTAWALGN YHRFFRLYCH
721APCMSGYLVD KFADRERKVA LKAMIKTFRP ALPVSYLQAE LAFEGEAACR AFLEPLGLAY
781TGPDNSSIDC RLSLAQLSAF
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

800 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.