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LEMD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

LEMD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all LEMD2 antibodies →
$504.00each
Catalog Number: LS-G104539-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LEMD2 (NM_181336), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ482C21.1 | HLEM2 | LEM domain containing 2 | LEMD2 | NET25
Origin Species
Human
NCBI GenBank
AK024858
SwissProt
Q8NC56

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGLSDLELR RELQALGFQP GPITDTTRDV YRNKLRRLRG EARLRDEERL REEARPRGEE
61RLREEARLRE DAPLRARPAA ASPRAEPWLS QPASGSAYAT PGAYGDIRPS AASWVGSRGL
121AYPARPAQLR RRASVRGSSE EDEDARTPDR ATQGPGLAAR RWWAASPAPA RLPSSLLGPD
181PRPGLRATRA GPAGAARARP EVGRRLERWL SRLLLWASLG LLLVFLGILW VKMGKPSAPQ
241EAEDNMKLLP VDCERKTDEF CQAKQKAALL ELLHELYNFL AIQAGNFECG NPENLKSKCI
301PVMEAQEYIA NVTSSSSAKF EAALTWILSS NKDVGIWLKG EDQSELVTTV DKVVCLESAH
361PRMGVGCRLS RALLTAVTNV LIFFWCLAFL WGLLILLKYR WRKLEEEEQA MYEMVKKIID
421VVQDHYVDWE QDMERYPYVG ILHVRDSLIP PQSRRRMKRV WDRAVEFLAS NESRIQTESH
481RVAGEDMLVW RWTKPSSFSD SER
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

503 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.