Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LDHD / Lactate Dehydrogenase D Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G103894-100
Catalog Number: LS-G103894
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LDHD (NM_194436), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- D-lactate dehydrogenase | LDHD | Lactate dehydrogenase D
- Origin Species
- Human
- SwissProt
- Q86WU2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MARLLRSATW ELFPWRGYCS QKAKGELCRD FVEALKAVVG GSHVSTAAVV REQHGRDESV61HRCEPPDAVV WPQNVEQVSR LAALCYRQGV PIIPFGTGTG LEGGVCAVQG GVCVNLTHMD121RILELNQEDF SVVVEPGVTR KALNAHLRDS GLWFPVDPGA DASLCGMAAT GASGTNAVRY181GTMRDNVLNL EVVLPDGRLL HTAGRGRHFR KSAAGYNLTG LFVGSEGTLG LITATTLRLH241PAPEATVAAT CAFPSVQAAV DSTVHILQAA VPVARIEFLD EVMMDACNRY SKLNCLVAPT301LFLEFHGSQQ ALEEQLQRTE EIVQQNGASD FSWAKEAEER SRLWTARHNA WYAALATRPG361CKGYSTDVCV PISRLPEIVV QTKEDLNASG LTGSIVGHVG DGNFHCILLV NPDDAEELGR421VKAFAEQLGR RALALHGTCT GEHGIGMGKR QLLQEEVGAV GVETMRQLKA VLDPQGLMNP481GKVLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
484 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.