Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
LDHA / LDH1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128074-100
Catalog Number: LS-G128074
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LDHA (NM_001165414), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LDH muscle subunit | LDH1 | LDHA | Lactate dehydrogenase M | LDH-M | GSD11 | Lactate dehydrogenase A | LDH-A | LDHM | PIG19 | Proliferation-inducing gene 19
- Origin Species
- Human
- NCBI GenBank
- NM_005566
- SwissProt
- P00338
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGEPSGGYTY TQTSIFLFHA KIPFGSKSNM ATLKDQLIYN LLKEEQTPQN KITVVGVGAV61GMACAISILM KDLADELALV DVIEDKLKGE MMDLQHGSLF LRTPKIVSGK DYNVTANSKL121VIITAGARQQ EGESRLNLVQ RNVNIFKFII PNVVKYSPNC KLLIVSNPVD ILTYVAWKIS181GFPKNRVIGS GCNLDSARFR YLMGERLGVH PLSCHGWVLG EHGDSSVPVW SGMNVAGVSL241KTLHPDLGTD KDKEQWKEVH KQVVESAYEV IKLKGYTSWA IGLSVADLAE SIMKNLRRVH301PVSTMIKGLY GIKDDVFLSV PCILGQNGIS DLVKVTLTSE EEARLKKSAD TLWGIQKELQ361FTRTRPLEQKLISEEDLAANDILDYKDDDDKV
361 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.